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Water-borne Yersinia enterocolitica from India
Dr. J.S. Virdi and his co-workers, Department of Microbiology, University of Delhi are working with emerging water-borne pathogens and their prevalence in the environmental waters of India. Their studies have shown the presence of Yersinia enterocolitica, an important food and water-borne pathogen, in wastewater, river water and ground water samples collected from Delhi and its surrounding areas. However they failed to isolate other emerging water borne pathogens such as Vibrio cholerae 0139 and E. Coli 0: 157, H7 in the environment waters. Many of the strains isolated by them are registrants to arsenic and cadmium. These isolates obtained from India also show certain unique antibiotic susceptibility profiles not shown by those isolated from other parts of the world. Currently they are doing DNA fingerprinting of Indian isolates of Y. enterocolitica to see their relationship with strains isolated from other parts of the world. An array of DNA fingerprinting technique viz. ribotyping; 168-238 intergenic spacer region ribotyping, REP (repetitive extragenic palindome) and ERIC (enterobacterial repetitive intergenic consensus sequence) are being used to achieve this goal. All isolates of Y. enterocolitica isolated from India have been authenticated by Yersinia Reference Laboratory and WHO Collaborating Centre at Pasteur Institute, Paris (France). All of their bacterial strains were deposited with the National repository i.e. Microbial Type Culture Collection (MTCC) and Gene Bank located at Institute of Microbial Technology (IMTech), Chandigarh. Each isolate has been given a separate accession number and can be retrieved by any prospective investigator who wishes to work on Indian strains of Y. enterocolitica.
(Contact: Dr.J.S.Virdi, Reader, Department of Microbiology, University of Delhi South Campus, Benito Juarez Road, New Delhi - 110021. e-mail: virdi_dusc@rediffmail.com).
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