Dr.
J.S. Virdi and his co-workers, Department of Microbiology,
University of Delhi are working with emerging
water-borne pathogens and their prevalence in
the environmental waters of India. Their studies
have shown the presence of Yersinia enterocolitica,
an important food and water-borne pathogen, in
wastewater, river water and ground water samples
collected from Delhi and its surrounding areas.
However they failed to isolate other emerging
water borne pathogens such as Vibrio cholerae
0139
and E. Coli 0:
157,
H7 in the environment waters. Many of the
strains isolated by them are registrants to arsenic
and cadmium. These isolates obtained from India
also show certain unique antibiotic susceptibility
profiles not shown by those isolated from other
parts of the world. Currently they are doing DNA
fingerprinting of Indian isolates of Y. enterocolitica
to see their relationship with strains isolated
from other parts of the world. An array of DNA
fingerprinting technique viz. ribotyping; 168-238
intergenic spacer region ribotyping, REP (repetitive
extragenic palindome) and ERIC (enterobacterial
repetitive intergenic consensus sequence) are
being used to achieve this goal. All isolates
of Y. enterocolitica isolated from India
have been authenticated by Yersinia Reference
Laboratory and WHO Collaborating Centre at Pasteur
Institute, Paris (France). All of their bacterial
strains were deposited with the National repository
i.e. Microbial Type Culture Collection (MTCC)
and Gene Bank located at Institute of Microbial
Technology (IMTech), Chandigarh. Each isolate
has been given a separate accession number and
can be retrieved by any prospective investigator
who wishes to work on Indian strains of Y.
enterocolitica.
(Contact: Dr.J.S.Virdi,
Reader, Department of Microbiology, University of
Delhi South Campus, Benito Juarez Road, New Delhi
- 110021. e-mail: virdi_dusc@rediffmail.com).
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