001. Negar Moghimian, Seyed Mohsen Hosseini, Yahya Kooch, Behrouz Zarei Darki. Faculty of Natural Resources & Marine Sciences, Tarbiat Modares University, 46417-76489 Noor, Mazandaran, Iran. Evaluating soil biochemical/microbial indices as ecological indicators of different land use/cover in northern Iran. Acta Ecologica Sinica, 2019, Vol. 39 (4), Pages: 328 - 333.

      Microbial community structure plays a significant role in the efficiency of dark fermentative hydrogen production using mixed culture. However, the detailed evolutions in microbial community structure during dark fermentation process are still unclear. This study investigated the detailed evolution patterns of microbial community structure during dark fermentation process by high-throughput pyrosequencing. Results showed that microbial community structure changed significantly over time in dark fermentation. Microbial diversity showed a constant decreasing trend during the fermentation process. The analysis of microbial community composition showed that Clostridium sensu stricto 1, Paraclostridium, Romboutsia and Paeniclostridium, which were all rarely existed in the inoculum, dramatically became dominant genera in the system after 6 h fermentation, with total relative abundance of more than 99%. This interesting result revealed that how quickly hydrogen-producing genera overwhelmed the microbial community in dark fermentation. Spearman correlation analysis showed that Clostridium sensu stricto 1 contributed the most to hydrogen fermentation performances.

Keywords: Biohydrogen production; Dark fermentation; High-throughput pyrosequencing; Microbial community; Evolution pattern.