9 2 g
Home About us MoEF Contact us Sitemap Tamil Website  
About Envis
Whats New
Microorganisms
Research on Microbes
Database
Bibliography
Publications
Library
E-Resources
Microbiology Experts
Events
Online Submission
Access Statistics

Site Visitors

blog tracking


 
Biochemical Engineering Journal
Vol. 100, 2015, Pages: 41–49

Systematic methodology for bioprocess model identification based on generalized kinetic functions

Anne Richelle, Philippe Bogaerts

3BIO-BioControl, Université Libre de Bruxelles, Avenue F. Roosevelt, 50, CP 165/61, 1050 Brussels, Belgium.

Abstract

This study presents a new systematic methodology for kinetic model identification on the basis of available experimental measurements. In a first step, a general kinetic model [Syst. Anal. Model. Simul. 35 (1999) 87–113] is identified, which allows, on the one hand, capturing the activation and/or inhibition effects of any component involved in the reaction and, on the other hand, identifying all the parameters based on a simple linear regression. This circumvents the tedious problems of choosing the kinetic structure and providing initial parameter values on a trial-and-error basis. In a second step, the general kinetic model can be easily transformed into the general extended Monod formalism. The global identification of the nonlinear model is finally performed based on the results of the previous steps. The model and the experimental field (fed-batch culture experiments using hybridoma cell line HB-58) presented in Amribt et al. [Biochem. Eng. J. 70 (2013) 196–209] are used as case study to underline the advantages of this strategy for the global identification of nonlinear models. The proposed systematic procedure leads to the identification of a model structure with similar complexity but whose parameter values present lower variation coefficients. The identified model successfully reproduces the dynamics associated with substrates consumption (glucose and glutamine), metabolites production (lactate and ammonia) and cell growth.

Keywords: Dynamic modeling; Fed-batch culture; Hybridoma cultures; Kinetic parameters; Production kinetics; Substrate inhibition.

 
Copyright © 2005 ENVIS Centre ! All rights reserved
This site is optimized for 1024 x 768 screen resolution