Department of Biotechnology, Dravidian University, Kuppam, A.P, India.
Abstract
Objective
To assess the probiotic nature of Lactobacillus in preventing cervical pathogens by studying the effectiveness of antimicrobial activity against vaginal pathogens.
Methods
Lactobacilli were isolated from healthy vaginal swabs on selective media and different pathogenic bacteria were isolated by using different selective media. The Lactobacillus strains were tested for the production of hydrogen peroxide and antimicrobial compounds along with probiotic properties.
Results
Of the 10 isolated Lactobacillus strains, strain 1, 3 and 6 are high hydrogen peroxide producers and the rest were low producers. Results of pH and amines tests indicated that pH increased with fishy odour in the vaginal fluids of cervicovaginal infection patients when compared with vaginal fluids of healthy persons. The isolates were found to be facultative anaerobic, Gram-positive, non-spore-forming, non-capsule forming and catalase-negative bacilli. The results of antimicrobial activity of compounds indicated that 280 and 140 μg/mL was the minimum concentration to inhibit the growth of both pathogens and test organisms respectively.
Conclusions
The results demonstrated that Lactobacillus producing antimicrobial compounds inhibits the growth of cervical pathogens, revealing that the hypothesis of preventing vaginal infection by administering probiotic organisms has a great appeal to patients, which colonize the vagina to help, restore and maintain healthy vagina.
Environmental Information System (ENVIS) provides national and international
environmental information in the disciplines of pollution control,microorganisms,envis,
toxic chemicals, central and offshore ecology, environmentally sound and appropriate
technology, bio-degradation of wastes and environment management.
microorganims,envis,environment information system centre, microorganisms
and environment management, environment management, microorganisms, microbes
Environment Information System Centre - microorganims,envis,environment
management, microorganisms, microbes, pollution control, toxic chemicals,
central and offshore ecology, environmentally sound and appropriate technology,
bio-degradation of wastes.